Part:BBa_K325209

Green Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)

This is the wild type luciferase from Luciola cruciata with a luciferin regenerating enzyme both under the control of an arabinose induced promoter.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 3044
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  Illegal NheI site found at 3278
  Illegal SpeI site found at 3044
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  Illegal BamHI site found at 2169
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 3044
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 3044
  Illegal AgeI site found at 979
  Illegal AgeI site found at 2118
  Illegal AgeI site found at 3820
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  Illegal SapI.rc site found at 1550
  Illegal SapI.rc site found at 3334